Pre-installed bio-mass Single-Use-Sensor
Capacitance sensor technology measures equally well adherent or suspension type of mammalian cells during cultivation. Typical range for mammalian is 105 cells/ml to 109 cells/ml.
The bio-mass sensor system sense total capacitance measured in pico Farad/cm with 2-3% accuracy. Viable Cell Density (VCD) system monitors the volume of live cells. The principle is expressed in permittivity as the system can detect the response of cells with intact membranes. Cell fragments, particles and gas bubbles are not measured.
The new Hamilton Incyte-P-SU biomass patch is suitable for attachment on cylindrical ridged plastic CerCell SUB sidewall and the easily attached pre-amplifier pointing upwards. From left the patches and the Incyte-W-ARC sensor amplifier. Centre photo show the 3.2 liter CellVessel Single-Use-Bioreactor equipped with Incyte-W-ARC, VisiWell and OneFerm-ARC Single-Use-Sensors. Right photo show the 13 liter CellVessel SUB mounted with Incyte-W sensor. Plus the Sipylus VP cable and a Cilix drive motor both from www.cronus-pcs.com.
Measured parameters on mammalian cell (app 18 µm size) are: Viable Cell Density, Conductivity, Fc, indicating average cell size, Alpha, indicating cell size distribution, DeltaEpsilon, 50 kHz to 20 MHz multi frequency cell density.
Photo show InCyte RUS installed on the SUS patch on the 1,000 ml iSUB. A 3.2 liter CellVessel InCyte mounted on the SUS patch further show the VisiWell.
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