0,4 - 2 liter LowRider and Levitronix

PR-SUB (Perfusion-Ready-Single-Use-Bioreactor)

Take advantage of a fully pre-assembled perfusion systems integrating the “LowRider-2-TFF” PR-SUB and save hood and autoclaving time. Bottom broth outlet connected directly to the Levitronix Single-Use-Pump (SUP) for circulating the broth through the Hollow-Fiber-Filter (HFF) and back to the SUB. The LowRider-2-TFF is designed to fit any 0,4-2 liter Working Volume (WV) perfusion Process-Control-System (PCS). Broadly known as TFF setup.

The LowRider-2-TFF PR-SUB with the unique broth outlet at bottom via a hose directly connected to the Levitronix SUP. Simple connection to the HFF follow by the retentate return through a hose and back into the LowRider-2-TFF.

Features for the configurable LowRider-2-TFF size PR-SUB

  • First class rigid mono component PolyCarbonate components
  • Any colour on head-plate (light blue is standard)
  • Dimension: OD 137 x 270 mm vessel
  • Magnetic-Bottom-Drive (MBD) for impeller integrated with Aero magnet motor:
    1. Radial agitation Elephant ear impeller, OD 60 mm, 3 blades, 45 degrees, CW rotation down flow, 55 mm from the bottom = traditional design
    2. Axial agitation inside cylindrical stator, Elephant ear impeller, OD 60 mm, 3 blades, 45 degrees, CW rotation down flow, 55 mm from the bottom and stator system = lower RPM and reduced foam production - check more info on this link
  • 1 x PG 13,5 port pre-installed with PolarWell or VisiWell for non-invasive DO Re-Usable-Sensor (RUS) insert
  • 1 x PG 13,5 port pre-installed with polymeric pH Single-Use-Sensor with K9 connector
  • 3 x free / extra sensor ports PG 13,5 - check out head plate details on this link
  • 1 x L-sparger macro or your preferred sparger
  • 1 x deep tube for retentate return with 500 mm 10/14 hose
  • 1 x “LowRider” barb broth outlet + 200 mm long 9,5/14,3 hose to the Levitronix PuraLev SUP
  • 1 x PuraLev SUP arranged with 3/4" Tri-Clamps exit to the HFF 
  • 1 x HFF of your choice
  • 6 x small inner diameter 900 mm long weldable hoses for various inlet and outlets on head plate
  • 1 x bottom harvest with swabable valve
  • 1 x exhaust with 2 x OD 60 mm sterile filters

Needed or nice to have re-usable accessories

  • Re-Usable electrical heating jacket (pn 2266-E) to be bolted on the MST = very safe solution
  • Re-Usable flexible electrical heating blanket (dim 75 x 350 mm) = traditional setup
  • Magnetic-Stirrer-Table (MST) like the Compact model (p/n: 2250-C-8-B) = any servo motor
  • SUB support plate for MST (p/n: 2259-137-15) = use with electrical heating blanket
  • If needed - converter kit for servo motor (p/n: 2252, p/n: 2253, p/n: 2254)
  • If needed - Sterile-Filter-Heater (p/n: 2211)
  • Accessories to be found here - Accessory Products

Photos with pre-installed PuraLev Single-Use-Pump from Levitronix, hoses and HFF on the LowRider-2-TFF PR-SUB. All ready to use right our of the dual foil bag in the TFF setup.

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