Bio-mass sensors in general

Capacitance sensor technology

Capacitance sensor technology measures equally well adherent or suspension type of mammalian cells. The bio-mass sensor system senses total capacitance measured in pico Farad. Viable Cell Density (VCD) system monitors the volume of live cells. The principle is expressed in permittivity as the system is capable of detecting the response of cells with intact membranes. Cell fragments, particles and bubbles are not measured.

Capacitance biomass Re-Usable-Sensors (RUS) are available in PG13.5 x 120, 225, 325 mm length. The sensor communicates on-line and only through the specific pre-amplifier to a computer/display and various output for external devices. The RUS, pre-amplifier and display is available from:

www.hamiltoncompany.com/products/process-analytics

www.aberinstruments.com

Measured parameters are: Viable Cell Density, Conductivity, Fc, indicating average cell size, Alpha, indicating cell size distribution, DeltaEpsilon, multi frequency cell density.

Biomass test performed in CellTank

Under references on this website you will notice that as much as 200 pF/cm has been observed in CellTank #34-0150 cultivating mammalian CHO suspension cell lines. In general the conversion ration is:

  • Approximately 1 pF/cm is equivalent to 1x10E+06/ml cells for suspension cells
  • Approximately 10 pF/cm is equivalent to 1x10E+06/ml cells for adherent cells

Measured at 37°C temperature. We recommend reading the KTH March 2012 presentation found under http://perfusecell.com/download